mouse anti αsma  (Progen Biotechnik)

Journal: bioRxiv

Article Title: A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal Transition

doi: 10.64898/2026.04.14.718463

Figure Lengend Snippet: (a-c) SM22α, eNOS, αSMA, and Fibronectin (FN) mRNA levels determined by qRT-PCR in human coronary artery endothelial cells (HCAEC) following treatment with TGFβ1 (10 ng/ml, 7d), hypoxia (5% O 2 , 4d) or TGFβ1 plus hypoxia (4d), in the absence or presence of ethanol (EtOH) at concentrations indicated (0-100 mM range). (d) SM22α, eNOS, and Cdh5 mRNA levels in HCAEC treated with TGFβ1 (10 mg/ml, 2d) +/- EtOH 25 mM or 100 mM. (e) Representative western blots showing CD31, Cdh5, SM22α, and SNAIL protein expression in HCAEC treated with TGFβ1, IL-1β, or Hypoxia +/- EtOH (25 mM or 100 mM). β-actin or GAPDH were used as loading controls. Data are mean±SEM, n=3. *p<0.05 vs control (no treatment), #p<0.05 vs TGFβ1 or hypoxia.

Article Snippet: Primary antibodies used include human CD31 (Cat. # AF806, R&D Systems), αSMA (MAB1420, R&D systems), SM22α (Cat. # 36090 CST), and CDH5 (Cat. # 2500, CST).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: bioRxiv

Article Title: A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal Transition

doi: 10.64898/2026.04.14.718463

Figure Lengend Snippet: (a) CD31 expression in HCAEC treated with TGFβ1 (10ng/ml), Hypoxia (5% O 2 ), or TGFβ1+ Hypoxia in the absence or presence of moderate dose ethanol (EtOH 25 mM). (b) αSMA expression in HCAEC treated with TGFβ +/- either moderate dose ethanol (25 mM EtOH) or high dose ethanol (100 mM EtOH). (c) SM22α and (d) αSMA expression in HUVEC treated with IL1β+TGFβ2 in the absence or presence of either 25 mM EtOH or 100 mM EtOH as indicated. Representative immunofluorescence images, from at least 3 experiments, shown.

Article Snippet: Primary antibodies used include human CD31 (Cat. # AF806, R&D Systems), αSMA (MAB1420, R&D systems), SM22α (Cat. # 36090 CST), and CDH5 (Cat. # 2500, CST).

Techniques: Expressing, Immunofluorescence

Journal: bioRxiv

Article Title: A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal Transition

doi: 10.64898/2026.04.14.718463

Figure Lengend Snippet: (a) (b) Representative images of immunofluorescently stained carotid cross sections from sham-operated and ligated controls, moderate EtOH, and Binge EtOH experimental groups (males). Blue = Dapi nuclear stain, red = Tm-Cdh5+, and white=αSMA+. (c) Cells co-expressing Cdh5 and αSMA (i.e., myo-endothelial cells indicative of EndMT) were quantified using QuPath bioimage analysis software in carotid cross sections post-ligation from controls (grey bars), moderate EtOH (green bars), and binge EtOH (red bars) experimental groups. Bar graphs show cumulative data expressed as % Myoendothelial cells /full cross section, % Myoendothelial cells /neointima, or Number of myoendothelial cells/full cross section. Data are mean±SEM, n=25-30 sections from 5-6 mice). *p<0.05, ** P<0.001, **** p<0.0001.

Article Snippet: Primary antibodies used include human CD31 (Cat. # AF806, R&D Systems), αSMA (MAB1420, R&D systems), SM22α (Cat. # 36090 CST), and CDH5 (Cat. # 2500, CST).

Techniques: Staining, Expressing, Software, Ligation

Journal: Cancer Imaging

Article Title: Preclinical evaluation of FAP-targeted PET imaging to investigate CAF responses to radiotherapy

doi: 10.1186/s40644-026-01010-2

Figure Lengend Snippet: Ex vivo analyses of tumor-infiltrating CAFs by flow cytometry and immunohistochemistry. A ) Immunohistochemistry micrographs of excised LLC and CT26 tumors, showing intratumoral αSMA+ CAFs (brown color) from tumor samples retrieved 1 week post-RT. The arrows indicate areas in the tumor periphery with enhanced aSMA+ CAF staining. B ) Digitally quantified +αSMA expression on whole tumor tissue slides ( n = 6 per strain). C ) Flow cytometry analyses to quantify FAP+ cells in enzymatically digested fresh tumor tissue samples retrieved 1 week post-RT. The bars represent the mean values ± SDs. Sham-irradiated tumors (non-irradiated) and tumors treated with 2x6Gy and 1 × 12 Gy were compared ( n = 6). Statistics: one-way ANOVA followed by Dunnett post hoc corrections for multiple comparisons

Article Snippet: An anti-mouse αSMA antibody (D4K9N, Cell Signaling) for identifying and quantifying CAF levels in tumors was used at a 1:100 dilution for staining.

Techniques: Ex Vivo, Flow Cytometry, Immunohistochemistry, Staining, Expressing, Irradiation